DNA

Part:BBa_K5061007:Design

Designed by: Florent Cha   Group: iGEM24_Evry-Paris-Saclay   (2024-09-14)


Golden Gate adapter for Test Plasmid (TP) Golden Gate cloning platform


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 18
    Illegal BsaI.rc site found at 6


Design Notes

two BsaI recognition sites in opposite direction


Source

DNA synthesis

References